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split samples that were tested by MVTL is provided on Table 4 in Appendix B. Copies of <br />MVTL's analytical reports are contained in Appendix C. <br /> <br />The split-sample data indicates var/ability in the samples results. Relative percent differences <br />(RPD) ranged from 29% to 162%. Presumably, this is not atypical for fecal samples because of <br />the potential lack of uniformity of the sample resulting in potential uneven distribution of waste <br />matter in the sample matrix. Th.is lack of uniformity can be ~rther compounded by split <br />sampling (dividing or splitting a sample for analysis by two or more laboratories) and laboratory <br />preparation and analyses procedures. Fecal matter also has a relatively short life-span so natural <br />decay can also effect results. Overall, the split results continued concentrations of fecal coliform <br />above the 200 CFU/100 ml standard. <br /> <br /> 3.6 FECAL STREPTOCOCCI DATA <br /> <br />On behalf of the City, Liesch requested MVTL to test for fecal streptococci in an attempt to <br />differentiate between human or animal waste origin. Copies of their analytical reports are <br />contained in Appendix C and the results area summarized on Table 5 in Appendix B. <br /> <br />Fecal coliform (FC) / fecal streptococci (FS) ratio's greater than 4.0 were once considered <br />indicative of human fecal contamination, whereas a ratio less than 0.7 was suggestive of <br />Contamination by' non-human sources. For various reasons, including survival rates of fecal <br />streptococci group species, the FC/FS ratio assessment has a high false-positive rate and <br />therefore should not be used as the sole means of differentiating between human and non-human <br />sources of pollution (Appendix D). Although this ratio is no longer relied upon in and of itself, it <br />could provide a general means of potentially identifying primary waste type. However, the <br />abnormally high precipitation associated with the sampling period allows for greater mixing of <br />waste types that may enter surface waters. This should be taken into consideration when <br />evaluating the data. . <br /> <br />Overall, the results of the testing were inconclusive. Generally, FC/FS ratio's were higher at <br />Station 16 (Big Elk Lake Outlet) and lowest at Station 27 (downstream of feedlot) and at the <br />Orono Beach. This data is suggestive of animal waste impacts near the feedlot and beach. <br /> <br />3.7 MISCELLANEOUS INFORMATION / DATA <br /> <br />Other information provided to Liesch for review included the following: <br /> <br />City of Elk River Memorandum dated September 4, 2002 - Information provided in the <br />memorandum indicated that on August 5, 2002 City of Elk River and Sherbume County <br />Environmental personnel investigated a complaint fi-om the Sherbume County SWCD <br />concerning possible fecal coliform bacteria contamination of the Elk River from feedlots <br /> <br /> LIESCH ASSOCIATES, INC. <br />Hydrogeologists · Engineers · Environmental Scientists <br /> <br /> · Page 5 · <br />· Jan-2003 · <br /> <br /> <br />