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<br />Elk River Parks & Recreation <br />Indoor Air Quality .A.ssessment <br />lEA Project No. 7805-1301 <br /> <br />Page 3 <br /> <br />Table 1: Concentrations of Culturable Airborne Fungi - May 9, 2006 (continued) <br /> <br />Sample N 0./ Agar Fungal Identification Colony Cone. Percentage <br />L.ocation Used Counts (CFU/m3) (%) <br />050906BR-03 DG-l 8 Aspergillus versicolor 117 1,376 90 <br />Cubicle Area CI adosporiu/ll 9 106 7 <br /> sterile fungi 3 35 2 <br /> Alternaria 1 12 <1 <br /> Total: 1,529 <br /> <br />CFU - Colony Fornling Unit: A distinct colony that fOnTIS on a sample plate <br />CFU/m3 - Colony forming units per cubic meter of air <br />DG-lS - Dichloran Glycerol Agar <br />Percentage - Percent of Total Organisnls Identified <br /> <br />Quick Reference Guideline: <br />Fungal organisms isolated in the indoor air sample are evaluated in comparison to the outdoor sample in tem1S of <br />type and concentration. Indoor concentrations should be lo\ver than outdoor concentrations in mechanically- <br />ventilated buildings \vith adequate filtration. Types of organisms found indoors should be sin1ilar to those found <br />outdoors. <br /> <br />. Discussion of Results <br /> <br />High airborne fungal levels \vere detected indoors in the cubicle area. Aspergillus versicolor \vas <br />the donrinant fungal organism \vhich is indicative of fungal gro\vth and contanrination due to <br />1110isture intrusion problen1S. Fungal gro\vih appears to be affecting indoor air quality. These <br />results indicate that an interior source of fungal contamination is present. The results of source <br />sanlpling (see belo\v) support this conclusion. <br /> <br />4.2 Fungal Contact Samples <br /> <br />Contact fungal samples \vere collected from the carpet bottom belo\v a chair nlat and along the <br />exterior \vall. Results of sampling are listed in the table belo\v: <br /> <br />Table 2: Contact Sanlple Fungal Concentrations - lVIay 9, 2006 <br /> <br />Sample No.1 Agar Fungal Identification Colony Percentage <br />Location Used Coun ts (0/0) <br />050906BR-04 DG-18 Aspergillus versicolor 72 100 <br />ReAnn's Cubicle - Bottom surface of Total: 72 <br />carpet belo\v chair n1at <br />050906BR-05 DG-18 Overloaded <500 N/A <br />rvlichelle's Office - Bottom surface of Aspergillus versicolor Total: <500 <br />carpet along exterior \vall <br /> <br />00-18 - Dichloran Glycerol Agar <br />Percentage - Percent of Total Organisms Identified <br />N/A - Not Applicable <br /> <br />Ouick Reference Guideline: <br />Contact samples \vith total colony forming units over 50 signify moderate fungal contan1ination and over 100 can <br />be considered heavily contaminated. The presence of potentially toxigenic or pathogenic fungi, such as <br />Staclzybotrys, Aspergillus or Penicilliu111 also is considered in evaluating fungal results. <br /> <br />. Discussion of Results <br /> <br />Moderate fungal counts \vere detected on the carpet bottonl sample collected belo\v the chair lllat <br />in the South\vest Cubicle. Aspergillus versicolor \vas the organism detected in the sanlple. The <br />elevated moisture levels found belo\v the chair mat suggest that moisture infiltration through the <br />slab \vas trapped by the chair mat and resulted in fungal gro\vih on the carpet. <br /> <br />The sample collected from the carpet bottom by the exterior \vall in the South\vest Office \vas <br />overloaded \vith Aspergillus versicolor. These results indicate that fungal gro\vth has occurred on <br />the carpet in the areas sampled. The visible fungal grO\vth behind the base cove on the exterior <br />belo\v grade \vall suggests that moisture has infiltrated through the exterior \vall and possibly the <br />slab in this area. <br /> <br />C:\Documents and Settings\tallard\Local Settings\Temporary Internet Files\OLKF\attach to 5 S.doc <br />